Objective:

Traditionally Aegle marmelos and Mucuna pruriens are applied on cuts and wounds. The present study was aimed at exploring the in vitro wound healing potential using CAM assay of Aegle marmelos and Mucuna pruriens and identification of active compounds that may be responsible for its wound healing action.

Methods:

Methanolic extract of Aegle marmelos leaves and Mucuna pruriens seeds were subjected to differential bioguided fractionation. Fractions were screened and flavonoid fraction was finally obtained for further investigation. HPLC analysis and spectroscopic techniques, including ultraviolet (UV) light, infrared (IR) light, mass (MS) and nuclear magnetic resonance (NMR) were used for identification and confirmation of bioactive compounds. The effects of isolated fractions of Aegle marmelos and Mucuna pruriens on number of new blood vessels and angiogenic inhibition (%) on chick embryo chorioallantoic membrane (CAM) were assessed.

Results:

Study revealed gallic acid (AM-3-03), quercitin (AM-4-01-01) and rutin (MP-4-03-01-01-01) compounds in the crude methanolic extract and bioactive compounds were identified and confirmed with standard gallic acid, quercitin and rutin using HPLC and UV spectroscopic methods. The isolated fractions promoted angiogenesis as evidenced by in vitro chick chorioallantoic membrane model. The isolated fractions were found to have better angiogenic properties from neovascularization studies. The results were statistically significant when compared to the control group (p < 0.05).

Conclusion:

These findings suggest that bioactive fraction of Aegle marmelos and Mucuna pruriens may enhance faster wound healing in vitro.